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Rabbit Biotechnology - Rabbit genomics, transgenesis, cloning and models
of: Louis-Marie Houdebine, Jianglin Fan
Springer-Verlag, 2009
ISBN: 9789048122271 , 136 Pages
Format: PDF, Read online
Copy protection: DRM
Contents
5
Chapter 1 Introduction
9
Chapter 2 Improvement of Rabbit Production
11
2.1 Introduction
11
2.2 Artificial Insemination (AI)
12
2.3 Induction of Parturition by Oxytocin
16
2.4 Nursing of Suckling Pups
16
2.5 Storage of Rabbit Semen
17
2.6 Rabbit Semen Freezing
17
References
19
Chapter 3 Basic Methods for Experimental Rabbits
21
3.1 Introduction
21
3.2 Handling and Restraint
21
3.3 Compound Administration
24
3.4 Sampling Techniques
27
3.5 Anesthesiology
29
3.6 Euthanasia
30
Reference
30
Chapter 4 Useful Information for Rabbit Genes, Proteins, and Antibodies
31
4.1 Introduction
31
4.2 Rabbit Genome, cDNA, and Protein Sequences
31
4.3 Primary Antibodies Against Rabbit Proteins
32
4.4 Other Useful Websites for Rabbit Physiology
34
Chapter 5 General Physiology of Rabbits
35
5.1 Delivering and Nursing Physiology (Tables 5.1–5.3)
35
5.2 Behavioral and Sensory Physiology
37
5.3 Urinary Physiology (Table 5.4)
37
5.4 Digestive Physiology (Table 5.5)
38
5.5 Respiratory Physiology
38
5.6 Cardiac Physiology (Table 5.6)
39
5.7 Hematologic Values
39
5.8 Serum Biochemical and Enzyme Values
39
5.9 Bleeding System for Obtaining More Correct Physiological Data and Animal Welfare
40
References
42
Chapter 6 Rabbit Transgenesis
44
6.1 Introduction
44
6.2 Pronuclear Microinjection Methods for Rabbit Transgenesis
45
6.3 Basic Technique for Microinjection
45
6.4 Other Possible Future Methods for Rabbit Transgenesis
50
References
52
Chapter 7 Rabbit as a Model for the Study of Human Diseases
55
7.1 Introduction
55
7.2 Studies Using Rabbit Models
56
7.3 Rabbit Models for Lipid Metabolism and Atherosclerosis
57
7.4 Rabbit Models for Other Human Diseases
61
7.5 Conclusions
65
References
66
Chapter 8 Transgenic Rabbits to Prepare Pharmaceutical Proteins
70
8.1 Introduction
70
8.2 Techniques to Generate Transgenic Rabbits
71
8.3 Production in Blood
72
8.4 Production in Milk
73
8.5 Quality of the Proteins Secreted in Milk
74
8.6 The Specific Properties of Rabbits as Living Fermentor
77
8.7 Conclusion and Perspectives
77
References
78
Chapter 9 Derivation and Characterization of Rabbit Embryonic Stem Cells: A Review
81
9.1 Introduction
81
9.2 Pluripotent Stem Cells of Different Origins
82
9.3 Rabbit Embryonic Stem Cells
84
9.4 Discussion
103
References
103
Chapter 10 Rabbit Cloning
109
10.1 Historical Background of Rabbit Biotechnology
109
10.2 Special Aspects of Rabbit Reproduction
110
10.3 State of Nuclear Transfer
112
10.4 Possible Applications of Rabbit Cloning
120
10.5 Appendix: Protocol for Rabbit Somatic-Cell Nuclear Transfer
122
Bibliography
124
Chapter 11 The European Rabbit Genome
133
Index
134
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